Journal: Scientific Reports

Article Title: A Lacticaseibacillus rhamnosus secretome induces immunoregulatory transcriptional, functional and immunometabolic signatures in human THP-1 monocytes

doi: 10.1038/s41598-024-56420-8

Figure Lengend Snippet: Conditioning with the LrS induces distinct morphological changes and increases the cell-surface expression of CD11b, CD11c, CX3CR1 and CD86 in THP-1 human monocytes. ( A ) Morphological confirmation of THP-1 monocyte differentiation into M2 macrophages following 72 h of conditioning with the LrS. Cells were stained with phalloidin-CF568 (red) and counterstained with DAPI (blue) ( B ) Histogram Overton subtraction was used to determine the relative number of cells expressing CD11b, CD11c, CD86, CX3CR1, or CD64 following conditioning with the LrS (red) over untreated (blue) controls. ( C ) Median fluorescence intensity (MFI) of THP-1 cells expressing CD11b, CD11c, CD86, CX3CR1, or CD64 was used to confirm the results of the histogram Overton subtraction. Data shown is the mean of the MFI ± SEM (n = 3). Significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 as determined by one-way ANOVA and Tukey’s post-hoc test.

Article Snippet: Anti-human FITC CD11c , Tonbo , 35-0116 , 3.9 , 0.2 μg/test.

Techniques: Expressing, Staining, Fluorescence

Journal: Scientific Reports

Article Title: A Lacticaseibacillus rhamnosus secretome induces immunoregulatory transcriptional, functional and immunometabolic signatures in human THP-1 monocytes

doi: 10.1038/s41598-024-56420-8

Figure Lengend Snippet: LrS and RA co-conditioning imprints an immunoregulatory profile in PD-THP-1 macrophages. Different letters denote significant differences ( p < 0.05) as determined by one-way ANOVA and Tukey’s post-hoc test. ( A ) Gene expression in PD-THP-1 macrophages conditioned with l -lactic acid, RA, LrS and LrS + RA. The mean fold change expression (mean ± SEM ( n = 3)) was normalized to baseline expression in PD-THP-1 macrophages. ( B ) Cytokine production in PD-THP-1 macrophages conditioned with l -lactic acid, RA, LrS and LrS + RA. Bar graphs represent mean cytokine production (mean (pg/mL) ± SEM ( n = 3)) for IL-10, IL-1Ra, % active TGF-β1, total TGF-β1 and IL-8. ( C ) Expression of cell surface markers on PD-THP-1 macrophages conditioned with l -lactic acid, RA, LrS and LrS + RA. The histograms and Overton subtraction values were generated using FlowJo software. Overton subtraction values represent the percentage of cells expressing CD83, CD80, CD86, CD64, CD103, CD209, CD11b and CD11c. Histograms represent one of three biological replicates. Bar graphs represent mean MFI values (mean MFI ± SEM ( n = 3)). ( D ) Morphological changes in PD-THP-1 macrophages induced by RA and LrS co-conditioning over 48 h; PD-THP-1 macrophages were then stained with phalloidin-CF488.

Article Snippet: Anti-human FITC CD11c , Tonbo , 35-0116 , 3.9 , 0.2 μg/test.

Techniques: Gene Expression, Expressing, Generated, Software, Staining

Journal: Scientific Reports

Article Title: A Lacticaseibacillus rhamnosus secretome induces immunoregulatory transcriptional, functional and immunometabolic signatures in human THP-1 monocytes

doi: 10.1038/s41598-024-56420-8

Figure Lengend Snippet: Antibodies used for flow cytometry analysis.

Article Snippet: Anti-human FITC CD11c , Tonbo , 35-0116 , 3.9 , 0.2 μg/test.

Techniques: Flow Cytometry, Concentration Assay